Host CD40 Is Essential for DCG Treatment Against Metastatic Lung Cancer.
نویسندگان
چکیده
BACKGROUND/AIM For the application of invariant natural killer T (iNKT) cells in cancer therapy, the CD40-CD40L interaction is indispensable in administering alpha-galactosylceramide (αGalCer). We hypothesized that CD40 plays an important role in dendritic cells (DC) pulsed with αGalCer (DCGs) in the treatment of lung metastases. MATERIALS AND METHODS Wild-type (WT) and CD40(-/-) mice were treated with DCGs isolated from WT or CD40(-/-) mice in a B16F10 lung metastases model and NK and NKT cell activity in lungs and the spleen were examined. RESULTS DCG treatment improved WT mice survival but CD40(-/-) hosts received no survival benefit. Conversely, attenuation of a therapeutic effect in mice treated with CD40(-/-) DCGs was not observed. The functional activities of NK and NKT cells in DCG-treated CD40(-/-) mice were partially suppressed. CONCLUSION Host CD40 is essential for DCG treatment to have a therapeutic effect on B16F10 lung metastases.
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عنوان ژورنال:
- Anticancer research
دوره 36 7 شماره
صفحات -
تاریخ انتشار 2016